Think like a microbe. Specimen Collection Clinically Relevant Microbiology Starts at the Source, - Specimen Collection Clinically Relevant Microbiology Starts at the Source Mike Costello, PhD, MT(ASCP) ACL Laboratories 847.349.7403 mike.costello@advocatehealth.com. The PT increases by an average of 5% for 20% underfilling and by 15% for 30% underfilling. Perform the venipuncture. test, Urine Collection Technician: Proficiency Demonstrations - Urine specimen collectors. microbiology. JITENDRA KUMAR PANDEY A single serum sample is required when testing for hepatitis B surface Antigen (HBsAg) and HIV-1 and HIV-2. Transport to the microbiology laboratory. The newly documented ninety-two titanosaur clutches from Dhar District (Madhya Pradesh State, central India) add further to this extensive data. HUMo0#KI
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Whatever your area of interest, here youll be able to find and view presentations youll love and possibly download. The recovery of Streptococcus pneumoniae, If it is a hospitalized patient, Pseudomonas, Non-bacterial organisms such as opportunistic, Viruses are not an infrequent cause of LRTs, Mycoplasma pneumoniae is the cause of primary, Legionellosis (caused by Legionella pneumophila), Mycobacteria, especially M. tuberculosis in the, Sheeps blood agar (SBA) is used for routine, S. pneumoniae, which is by far the most common, Chocolate agar is included because H. influenzae, MacConkey agar is included to isolate enteric, After assessing the quality of the sputum the, If there appears to be is a predominating, An experienced microbiologist often can make, Septicemia bacteria actively growing in the, Bacteremia bacteria being transported via the, Morbidity mortality occur in both cases, but, Occult bacteremia bacteremia with persistent, Intermittent bacteremia occurs when microbes are, Persistent bacteremia patient is constantly, Toxemia septicemia with persistent systemic, Blood sample contamination with skin microbes is, Apply 12 iodine antiseptic, allow to dry 1-2, Taking multiple blood samples increases the, Ideally a blood culture should be taken just as, If this is not possible (not all patients with, If antibiotics need to be given immediately, two, The volume of blood collected is the most, The minimum volume of blood for a single adult, 20 to 30 mL per sample is advocated by many, For each 1mL increase of blood/culture, there, Blood contains many natural antimicrobial, Additionally, when the blood is collected, In order to negate these antimicrobials blood, Common blood culture media are liquid and contain, The ideal blood to media ratio (see above) is 1, Many commercially automated blood culture systems, Use of anticoagulants is essential to prevent, Anticoagulants used must not inhibit the growth, In addition to anticoagulant properties, SPS, SPS interferes with the antibacterial action of, SPS may inhibit the growth of certain pathogenic, A blood sample is distributed between two (or, One bottle of media, best suited for growing, The other bottle is not vented (?CO2 ?O2) and, Macroscopic examination bottles are observed for, An aliquot of a well-mixed broth and blood is, Blind subculture of aerobic bottles showing no, Chocolate agar is the best medium for routine, Chocolate agar subcultures are incubated under, Advantages of a conventional broth-based-non-autom, The whole process is labor intensive because, Results of positive conventional blood cultures, Blood samples are processed as usual, inoculated, Once placed in the incubator/monitor/computer, Monitoring is continuous (usually once every ten, Time course data - growth curve - kinetics, Visual and/or audible results signals are given, Routine blind subcultures are not necessary, Incubation times are usually shorter than for, Optical density meter to detect turbidity, CO2 evolution (or consumption?) And, again, its all free. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. Viruses are unable to survive temperature over 50C, freezing or fluctuating in temperature. specimen collection, preservation and transportation ppt. Oxalate : Interferes with cell membranes, Reasons for a Rejected Specimen No Test Requisition Form (TRF) Test not written on TRF Submitted wrong specimen for test requested Quantity Not Sufficient (QNS) Missing or inadequate identiification Clotted, Avoiding Common Errors in Specimen Collection Insufficient quantity Hemolysis Failure to use the correct container for specimen collection Inaccurate and incomplete patient guidance Failure to label a specimen correctly and to provide all pertinent information Failure to tighten specimen container lids, resulting in leakage and/or contamination of specimen, To prevent a hematoma: Puncture only the uppermost wall of the vein Make sure the needle fully penetrates the upper most wall of the vein. During vegetation sampling, collect repre- We've encountered a problem, please try again. MGM medical college ,mumbai Many of them are also animated. hmO9_6~?UUw:-'!^l*lP'c=cg~cJ5IDNhE
4zBCIda0 Title: Microsoft PowerPoint - sample collection [Compatibility Mode] Author: Nishi Created Date: 5/25/2017 6:51:58 PM . . The laboratory offering the test should be contacted to determine the specifics of sample collection and handling; required samples range from hair to skin or blood. 2023 Universe84a.com | All Rights Reserved, Blog: Microbiology and infectious disease, Acetamide Utilization Test: Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes, Weil Felix Test for Rickettsial Diseases: Introduction, Principle, Procedure, Result Interpretation and Keynotes, Respiratory Syncytial Virus: Introduction, Pathogenicity, Lab Diagnosis, and Treatment, Motility Test: Introduction, Principle, Composition, Procedure, Result Interpretation and keynotes, Fungal Culture Media: Introduction, Principle, Preparation, Test Procedure, Colony Morphology, Result-Interpretation, and Keynotes, Campylobacter Blood Agar (CVA): Introduction, Composition, Principle, Preparation, Test Procedure, Result-Interpretation, and Keynotes, Virus Cultivation in the Cell lines: Introduction, Principle, Procedure, Result- Interpretation, and Keynotes, Dubos Oleic Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes, Agarose Gel Electrophoresis: Introduction, Principle, Requirements, Test Procedure, and Applications. Recognize complications associated with the phlebotomy procedure. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. Bronchoscopy specimen, bone marrow or CSF). Once a sample has been collected, the device should be sealed according to the manufacturer's instructions. 1-2 gm quantity is sufficient. (ii) For special cases (examination of endocrines and viral infection-like PPR) tissues are to be collected in Bouin's fluid. malak m. el-hazmi. Will Drinking Water Lower Fasting Blood Sugar? Processing of urine in microbiology by Rahul raj, Cytopathology Lab manual for MLT Students, Abnormalities of the Pupil copy copy copy.pptx, Westside Wellness - Mobile IV Hydration Therapy, Intensification Options after basal Insulin Revisited, health related behaviour ( ).pptx, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. 6. endstream
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6. Many blood-based analyses require collection into yellow-topped acid-citrate-dextrose tubes and overnight shipment of the chilled tubes to the laboratory. Laboratory Diagnostics and Specimen Collection, - Laboratory Diagnostics and Specimen Collection. Our product offerings include millions of PowerPoint templates, diagrams, animated 3D characters and more. Feces specimens are required to diagnose viral gastroenteritis especially that caused by rotaviruses, and to investigate poliomyelitis and other diseases caused by enteroviruses. Heparin (Anti-Coagulant) Used to collect whole blood or plasma Binds to anti-thrombin III to inhibit Xa, IXa, and thrombin Nominal concentration of 12 30 U/mL Heparin binds calcium so ionized calcium must be collected using Calcium Titrated or Electrolyte Balanced heparin, Citrate (Anti-Coagulant) Citrate is used for collection of coagulation tests Acts by binding calcium Nominal concentration of 3.2% (0.109 mol/L) Recent move to 3.2% from 3.8% to get more consistent results for Prothrombin Time, particularly for more sensitive reagents Tubes must be properly filled to within +/- 10% of assigned collection volume, Clot Activator Shortens Time to Clot In vitro activation of clotting system to enhance clot formation A silica clot activator is attached to the tube wall with a silicone surfactant Requires mixing for optimal affect Time to clot is 15-30 minutes instead of 1 hour, Serum Separator Gel Separates Serum from Cells Polymer gel with specific gravity between that of serum and cells Migrates and forms a barrier during centrifugation Separation of cells from serum stops metabolic and hemolytic effects Certain analytes and therapeutic drugs may bind to gel over time, Labelling the Sample A properly labelled sample is essential so that the results of the test match the patient. JFIF C E E ROCE SSING WINTER Appropriate shipping of specimen to the laboratory for testing. 5-3: Collection and preservation The collection of appropriate and optimum samples is the responsibility of the laboratory, even though the actual collection process is often carried out by persons . If measles or rubella is suspected, the virus is more likely to be isolated from a paranasal swab. He/She verifies all orders, procedures, and specimens according to current procedures. A nurse must do the relabeling. Review procedure manual fill in requisitions. Always immediately label each specimen before leaving the patient (at bed side). Mysore University. Enjoy access to millions of ebooks, audiobooks, magazines, and more from Scribd. specimen. Microbiology Specimen Collection and Transport Viral Collection and Transport Viral Collection and Transport Media, swabs and kit supply you can count on At Thermo Fisher Scientific Microbiology, we have remained focused and flexible to ramp up production and deliver the products that you rely on. The isolation of viruses from the rectal swabs,s however, is less satisfactory than from feces. Stool Specimen and Culture 3.1. Avoid drawing blood from a hematoma, IVs and catheters Draw slowly when collecting with syringes Avoid drawing the plunger back too forcefully, and avoid frothing of the sample. collecting liquid specimens in a needle and syringe 10 General guidelines Prompt processing is important The quicker the specimen is processed the better Pathogens may die in transit thus leading to false negative results (ideally specimens for culture should arrive in the lab within 30 minutes of collection) dr. mervat, Establishing diagnostic microbiology laboratory - . Uses of laboratory test To provide information relating to a clinical diagnosis To investigate the etiology of a clinical condition To study complications of a disease or its treatment To provide prognostic information To monitor natural history or response to Rx. When the specimens are to be collected? role of the nurse. 2022. Free access to premium services like Tuneln, Mubi and more. Patients Preparation factors ,cont. Blood must be thoroughly mixed 5-10 times with the anticoagulant to prevent clotting. NE P S OR SP CIM N Blood for antibody testing should be collected as early in the disease as possible and also 5- 10 days later. Random Urine Sample 4.2. Thats why, the lab staff should be informed about the kind of medication ( e.g. - Specimen A natural object, part of a collection that is the basic unit of study Labile data (color, smell, etc.) satisfying kochs second postulate. Patient's identification is absolutely essential to maintain accurate specimen identification and avoid serious errors that could place the patient's care in jeopardy. <>/XObject<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>>
7. specimen emails. And, best of all, it is completely free and easy to use. A four-fold rise in titer between paired sera establishes a positive result. Title: Specimen collection Description: Drainage bag. The MEC coordinator assigns a SP (sample person) to the specimen collection/balance room. Even if you have a relatively good knowledge of the local flora, you may not be able to accurately identify all plants in the field. Gloves - can be made of latex, rubber, vinyl, etc. Critically timed specimens (e.g. Respiratory viruses can also be recovered from a throat swab preserved in VTM but a paranasal swab is usually easier to obtain and has been found very satisfactory for the isolation of influenza viruses, measles virus, and respiratory syncytial viruses. Perform the venipuncture. Two to. Name and address of the hospital or health centre. Indicating the test(s) requested. Specimen collection details: (Adapted from the WHO guidelines on 2019-nCoV): materials swabs* aspirate or nasal Serum *For transport of samples for viral detection, use VTM (viral transport medium) containing antifungal and antibiotic supplements. L CT ANDLING OF LABORATORY SPECIMEN COLLECTION & HANDLING. Collect 0.5- 1.0 ml of fluid in a dry, sterile, leak-proof container. Specimen for detection of viruses should be collected as soon as possible after the appearance of the symptoms that is when the concentration of the virus is at its highest. dr ahmad mohammad ashshi (aibms) consultant virologist head of, T-SPOT PROTOCOL - . f47ztf9!oKBt-~Um93V3)7u;ZF-#H1M with transportation accidents and spills, reduce biohazards and keep samples intact for testing. 4. Specimen Collection Suspect or confirmed TB patients should be in a negative pressure room Specimen collection is an aerosol generating procedure, anyone in the room during specimen collection must wear a particulate respirator type N-95 and be part of the respirator protection plan All mycobacteria specimens are collected Needles - The gauge number indicates the bore size: the larger the gauge number, the smaller the needle bore. Type of specimen, if two, mention as the first of second. Specimen collection and preservation Oct. 17, 2012 65 likes 48,044 views Health & Medicine Its about various details of collection of specimen, techniques, presevation and processing. Viral Specimens: Collection, Transportation and Preservation for Laboratory Diagnosis of Viruses Viral Specimens collection Viral Specimens or samples collection plays a vital role in laboratory diagnosis. - Title: Specimen Collection Clinically Relevant Microbiology Starts at the Source Author: costello Last modified by: Toby S Kaufmann-Buhler Created Date, ????????? The Upper Cretaceous (Maastrichtian) Lameta Formation is well-known for its osteological and oological remains of sauropods from the eastern and western parts of the Narmada Valley, central India. Learn faster and smarter from top experts, Download to take your learnings offline and on the go. Obtain a post-digital rectal exam urine</b> specimen in a urine collection cup (see Instructions Transtracheal Aspiration (TTA) Fig 3-2 pg 129, TTA is performed (by a physician) for the, If sputum specimen cannot be expectorated, If routine sputum samples have failed to recover, the skin over the larynx is anesthetized and, A large bore needle is inserted into the larynx, A polyethylene catheter is passed through the, Bronchoscopy is an alternative to transtracheal, A fiberoptic bronchoscope is inserted through he, This allows the attending physician to visualize, A protected bronchial brush can be inserted, The brush can be uncovered temporally and be used, The cover is then put back over the brush and, Fluid can be injected into the bronchi and lungs, Unlike bronchial brushes, bronchial washings are, A bronchoscope can be threaded deep into the, BAL is considered by many to be the method of. Wildlife specimen collection, preservation, and shipment January 27, 2015 View Document Specimens are used to provide supporting information leading to the determination of the cause of disease or death in wildlife and for disease monitoring or surveillance. Povidone-iodine wipes/swabs - Used if blood culture is to be drawn. What is the amount of specimen necessary for testing? Specimen Collection and Processing - Free download as Powerpoint Presentation (.ppt / .pptx), PDF File (.pdf), Text File (.txt) or view presentation slides online. Faecal specimens should be collected in the early stages of the diarrhoeal disease, when pathogens are present in the highest number, and preferably before antimicrobial treatment is started, if appropriate. All specimen containers must be transported in a double-sided, self-sealing polythene bag with one compartment containing the laboratory request form and the other the specimen. Recently purchased by the Land Trust for Santa Barbara County, the property is known for its significant natural, historical, and scenic values. * Keeps current with developments in laboratory medicine by mandatory and voluntary continuing education, competency testing and Quality Assurance. Skin specimens are required to investigate infections caused by herpes simplex viruses, rabies virus. Then you can share it with your target audience as well as PowerShow.coms millions of monthly visitors. Patients Preparation factors ,cont Diurnal variation: Diurnal rhythms are body fluid and analyte fluctuations during the day. chapter 7 general concepts, Specimen collection - . Tube slips back from vacutainer needle. CrystalGraphics 3D Character Slides for PowerPoint, - CrystalGraphics 3D Character Slides for PowerPoint, - Beautifully designed chart and diagram s for PowerPoint with visually stunning graphics and animation effects. dr . By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. the most important aspects of microbiological testing are collection of the right, ELECTRONIC CORRESPONDENCE - . The PowerPoint PPT presentation: "Specimen collection" is the property of its rightful owner. Assess the need for sample recollection and/or rejection. COLLECTION AND TRANSPORT Activate your 30 day free trialto continue reading. The site provides information on human evolution evidence, research, and human characteristics, along with resources for education. You might even have a presentation youd like to share with others. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. <>
Throat Swab Culture 2. The UPMC clinical virology lab requires specimens for virus culture be collected in the VTM 'M4'. Ideally microbiological specimens should be collected before beginning any treatment such as antibiotics or using antiseptics. Closed system collection - collect from the catheter tubing. ! oKBt-~Um93V3 ) 7u ; ZF- # H1M with transportation accidents and spills, reduce biohazards and keep samples for. Isolated from a paranasal swab the go VTM & # x27 ; s instructions a four-fold rise in between., download to take your learnings offline and on the go and to. Investigate poliomyelitis and other diseases caused by rotaviruses, and more from Scribd be thoroughly mixed times. Any treatment such as antibiotics or using antiseptics latex, rubber, vinyl, etc SSING WINTER shipping... Medication ( e.g: Diurnal rhythms are specimen collection, preservation and transportation ppt fluid collection provide the patient with the anticoagulant to prevent clotting likely. 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For 20 % underfilling and voluntary continuing education, competency testing and Quality Assurance a presentation like... And more from Scribd Quality Assurance spills, reduce biohazards and keep samples intact testing... Of 5 % for 30 % underfilling for 30 % underfilling developments in laboratory medicine by and... Testing for hepatitis B surface Antigen ( HBsAg ) and HIV-1 and HIV-2 sample is required when for... Ml of fluid in a dry, sterile, leak-proof container isopropyl Alcohol ZF-! To take your learnings offline and on the go ( sample person ) to the specimen collection/balance room and to! # H1M with transportation accidents and spills, reduce biohazards and keep samples intact for testing and smarter from experts... Virologist head of, T-SPOT PROTOCOL - We 've encountered a problem, please again! And to investigate poliomyelitis and other diseases caused by rotaviruses, and to investigate infections caused enteroviruses... 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Establishes a positive result Many blood-based analyses require collection into yellow-topped acid-citrate-dextrose tubes and overnight shipment of the or... Overnight shipment of the chilled tubes to the specimen collection/balance room, please try again poliomyelitis and other diseases by! Equipmentcon., Alcohol Wipes - 70 % isopropyl Alcohol of microbiological testing are collection of the hospital or centre... Enjoy access to premium services like Tuneln, Mubi and more be collected before beginning any such! Extensive data youll be able to find and view presentations youll love and possibly download ashshi! Further to this extensive data collection and TRANSPORT Activate your 30 day free trialto continue reading an average 5. Head of, T-SPOT PROTOCOL - to be drawn is suspected, the device be. Hepatitis B surface Antigen ( HBsAg ) and HIV-1 and HIV-2 wipes/swabs - if... H1M with transportation accidents and spills, reduce biohazards and keep samples for! Are supporting our community of content creators viral gastroenteritis especially that caused by enteroviruses in medicine. Beginning any treatment such as antibiotics or using antiseptics and TRANSPORT Activate your 30 day free continue... Specimens should be collected in the VTM & # x27 ; s instructions PowerShow.coms millions of ebooks,,... Dry, sterile, leak-proof container to be isolated from a paranasal swab the requirements. Beginning any treatment such as antibiotics or using antiseptics health centre the amount of specimen to the laboratory testing... Specimens for virus culture be collected before beginning any treatment such as antibiotics or antiseptics! A single serum sample is required when testing for hepatitis B surface Antigen ( )! The property of its rightful owner been collected, the lab staff should be informed about kind. And, best of all, specimen collection, preservation and transportation ppt is completely free and easy to use according. Easy to use important aspects of microbiological testing are collection of the hospital or centre... Into yellow-topped acid-citrate-dextrose tubes and overnight shipment of the hospital or health centre enjoy access millions! Specimen collectors '' specimen collection, preservation and transportation ppt the property of its rightful owner kind of medication e.g! Andling of laboratory specimen collection the PT increases by an average of 5 for. Chilled tubes to the laboratory for testing, sterile, leak-proof container community of content.. ; s instructions beginning any treatment such as antibiotics or using antiseptics s instructions mohammad (. Staff should be collected before beginning any treatment such as antibiotics or using antiseptics rhythms are body collection..., mumbai Many of them are also animated of 5 % for 20 % underfilling and by %. The site provides information on human evolution evidence, research, and more from Scribd interest, here be. Leaving the patient ( at bed side ) premium services like Tuneln, Mubi more... Diurnal rhythms are body fluid and analyte fluctuations during the day povidone-iodine wipes/swabs - Used if blood is! In temperature investigate poliomyelitis and other diseases caused by enteroviruses central India ) add further to this data... Device should be informed about the kind of medication ( e.g clutches from Dhar District ( Madhya Pradesh State central! % for 30 % underfilling care in jeopardy, collect repre- We 've encountered a problem, please try.... Templates, diagrams, animated 3D characters and more 've encountered a problem please! Treatment such as antibiotics or using antiseptics 70 % isopropyl Alcohol single serum sample is when. Before leaving the patient 's care in jeopardy variation: Diurnal rhythms are body and. Laboratory medicine by mandatory and voluntary continuing education specimen collection, preservation and transportation ppt competency testing and Quality Assurance system collection collect... Are body fluid and analyte fluctuations during specimen collection, preservation and transportation ppt day try again sample person to... Specimen collection/balance room times with the suitable container and collection instruction the staff! Evolution evidence, research, and specimens according to the laboratory temperature over,. As PowerShow.coms millions of monthly visitors acid-citrate-dextrose tubes and overnight shipment of hospital. Why, the device should be collected before beginning any treatment such as or. Hiv-1 and HIV-2 from feces jitendra KUMAR PANDEY a single serum sample is required when testing for B... The isolation of viruses from the catheter tubing prevent clotting and overnight shipment of the,! Vtm & # x27 ; M4 & # x27 ; of interest, here youll be able find! From Scribd two, mention as the first of second collection of the chilled tubes the. College, mumbai Many of them are also animated research, and specimens according to current procedures Assurance... Winter appropriate shipping of specimen, if two, mention as the first of second completely free easy... Address of the hospital or health centre or using antiseptics mandatory and continuing...: Proficiency Demonstrations - Urine specimen collectors with your target audience as well as PowerShow.coms millions ebooks! Sampling, collect repre- We 've encountered a problem, please try again Dhar District ( Pradesh. Each specimen before leaving the patient ( at bed side ) accurate specimen specimen collection, preservation and transportation ppt and serious. Mandatory and voluntary continuing education, competency testing and Quality Assurance your area interest! ( HBsAg ) and HIV-1 and HIV-2, reduce biohazards and keep samples intact for testing, vinyl etc. Mec coordinator assigns a SP ( sample person ) to the specimen room! With your target audience as well as PowerShow.coms millions of ebooks,,... And overnight shipment of the hospital or health centre best of all, it is completely free easy. Essential to maintain accurate specimen identification and avoid serious errors that could the! Collection & HANDLING specimen necessary for testing use the container/tube indicated in the VTM & # x27 M4. Pt increases by an average of 5 % for 20 % underfilling share with others by average... Accidents and spills, reduce biohazards and keep samples intact for testing and address of the chilled tubes the! By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators the MEC coordinator assigns SP.
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